A genotypic method for determining HIV-2 coreceptor usage enables epidemiological studies and clinical decision support

Table 3 Results from the validation of the web service on nine additional V3 sequences

Isolate	FPR	Major markers	Minor markers	Visseaux prediction	geno2pheno[coreceptor-hiv2] prediction	Phenotype
ROD10 (Wildtype)	0.01	L18X, V3 net charge >6	NA	X4-capable	X4-capable	X4-capable
ROD10 (K29T)	0.01	L18X	NA	X4-capable	X4-capable	X4-capable
ROD10 (H18L)	0.03	V3 net charge >6	NA	X4-capable	X4-capable	X4-capable
ROD10 (H23Δ + Y24Δ)	0.01	L18X	NA	X4-capable	X4-capable	X4-capable
ROD10 (H18L + K29T)	0.03	NA	NA	R5*	X4-capable	X4-capable
ROD10 (H18L + H23Δ + Y24Δ)	0.11	V3 net charge >6	NA	X4-capable*	R5	R5
ROD10 (H18L + H23Δ + Y24Δ + K29T)	0.15	NA	NA	R5	R5	R5
15PTHSJIG	0.36	NA	NA	R5	R5	R5
15PTHCEC	0.01	L18X, V19K/R, Insertion24, V3 net charge >6	Q23R, R28K	X4-capable	X4-capable	X4-capable

Incorrect predictions are marked with an asterisk. ROD10 refers to the HIV2-group A reference strain, which uses both CCR5 and CXCR4 as entry coreceptors. Mutations from the ROD10 wildtype sequence are indicated in brackets, where Δ indicates deletions

ISSN: 1742-4690