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Fig. 9 | Retrovirology

Fig. 9

From: Membrane bound modified form of clade B Env, JRCSF is suitable for immunogen design as it is efficiently cleaved and displays all the broadly neutralizing epitopes including V2 and C2 domain-dependent conformational epitopes

Fig. 9

Cleavage property of JRCSF Env. a Cell surface staining assay of JRCSF and JRCSFSEKS with PG9, PGT151 and PGT145 over a range of antibody concentrations. b ELISA assay of gp120 shedding of JRCSF transfected 293T cells incubated with and without 50 μg/ml sCD4-183. c Western blot analysis of VRC01 immunoprecipitates of plasma membrane fraction of JRFL and JRCSF(N197D) transfected 293T cell lysates with anti-clade B Env rabbit Abs. d Western blot analysis of VRC01 immunoprecipitate of plasma membrane fraction of JRCSF(N197D) transfected 293T cell lysates and crude lysate of YU2 with anti-clade B Env rabbit Abs

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Retrovirology

ISSN: 1742-4690

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