HIV-1 clade C escapes broadly neutralizing autologous antibodies with N332 glycan specificity by distinct mechanisms

Table 3 Genetic and neutralization properties of autologous env genes

env clones	Clade	% identity^a	N332 genotype	PNLG V1 loop	PNLG V1V2 loop	Length V1 loop	Length V1V2 loop	Autologous^b neutralization (ID₅₀)	IC₅₀ ^c (µg/ml)
env clones	Clade	% identity^a	N332 genotype	PNLG V1 loop	PNLG V1V2 loop	Length V1 loop	Length V1V2 loop	Autologous^b neutralization (ID₅₀)	PGT121	PGT128
HIV-1 NLR-2007.J10	C	99.1	N332	5	10	41	96	36	>10	>10
HIV-1 NLR-2007.J12	C	100.0	N332	5	10	41	96	43	>10	>10
HIV-1 NLR-2007.J24	C	98.3	N332	5	10	41	96	83	>10	>10
HIV-1 NLR-2007.J32	C	99.5	N332	5	10	41	96	56	>10	>10
HIV-1 NLR-2007.J48	C	95.0	S332	2	07	27	76	25	>10	>10

^a % identity of the autologous env amino acid sequences to NLR-2007.J12 was deduced using LAALIGN tool (http://www.ch.embnet.org/software/LALIGN_form.html). It refers to the degree of correlation between two un-gapped sequences and indicates that the amino acid at the particular position is an exact match
^bID50 values refers to the reciprocal dilution of the plasma that mediated 50 % neutralization of the HIV-1 Env pseudotyped viruses in TZM-bl cell
^cIC50 values indicate concentration that mediated 50 % Env-pseudotyped virus neutralization in TZM-bl cells. Note that in our study, the highest mAb concentration used was 10 µg/ml

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