Fig. 4
APOL1 polymorphism affects IL-1β-dependent persistence of HIV-1 in CIHPs. a Time course experiments showing DC-SIGN gene expression in CIHPs after stimulation with rhIL-1β (25 ng/mL) compared to untreated cells (Ctrl) and normalized to GAPDH gene expression (N = 3). b Statistical histogram graph showing DC-SIGN gene expression after 18 h of rhIL-1β (25 ng/mL) treatment in CIHPs stably transfected with either empty vector (Ctrl) or WT or APOL1 risk Vs. Results are expressed as relative fold change in rhIL-1β treated cells versus non treated cells (Mock) and normalized to GAPDH gene expression (N = 3). c Statistical histogram graph showing HIV-1 accumulation after rhIL-1β stimulation in CIHPs stably transfected with the control vector (Ctrl) or the specific APOL1-WT or APOL1-G1/G2 expressing vectors. Results are expressed as relative fold change of HIV-1 DNA RU5 in HIV-1 incubated CIHPs versus non HIV-1-pulsed cells (Mock) and normalized to GAPDH gene (N = 3). d Rescue of infectious HIV-1 by CD4pos T lymphocytes co-cultured in time-course experiments (0, 5 and 7 days) with HIV-1 pulsed CIHPs stable transfected with control vector (Ctrl), APOL1-WT or APOL1-G1/G2 Vs and pretreated or not (Mock) with rhIL-1β. Results are expressed as fold increased of HIV-1 DNA Gag gene in PBMC co-cultured with HIV-1 pulsed CIHPs compared to their HIV-1 untreated counterparts and normalized to the amount of GAPDH gene (N = 3). Horizontal lines represent Mock and rhIL-1β in the Ctrl sample depicting the fold change in HIV-1 replication. e APOL1 gene expression in CIHPs incubated with rhIL-1β (25 ng/mL) and subsequently treated with HIV-1 for 6 h compared to their counterparts incubated with rhIL-1β alone, HIV-1 alone or untreated (Ctrl) Results are expressed as relative fold change normalized to GAPDH gene expression (N = 5). f Statistical histogram graph showing the APOL1 gene expression in CIHPs incubated with IL-1β (25 ng/mL) and subsequently treated with HIV-1 for 6 h either in pre absence (Ctrl) or in the presence of blocking anti-DC-SIGN mAb. Results are expressed as relative fold change in treated cells normalized to GAPDH gene expression (N = 3). P values *< 0.05, **< 0.01