Fig. 9

Viability and cell death in lymphocytes co-cultured with HIV-1 exposed FDCs. The FDC lines 1401 and 1402 were exposed to HIV-1 IIIB or SF162 o.n., washed and cultured for 2 days before PBMCs from healthy blood donors were added. The viability of lymphocytes was evaluated at 2 and 7 days of co-cultivation by Flow cytometry. The data represent the cumulative mean of vivid (a) negative lymphocytes in PBMCs from three donors co-cultured for either 2 or 7 days with FDCs. The viability of T (b) and B (c) cells is presented as cumulative mean and SD of vivid negative CD3+ and CD19+ positive cells in PBMCs from three donors co-cultured for either 2 or 7 days with FDCs. The levels of IgG (d) and IgM (e) in supernatants from PBMCs co-cultured with HIV-1 exposed and control FDCs for 10 days and measured by ELISA