Fig. 4
CCR6+DN proliferate in response to C.albicans but not CMV. a FACS-sorted T-cell subsets isolated from the peripheral blood of HIV-uninfected individuals were stained with CFSE, co-cultured with antigen-loaded autologous monocyte-derived dendritic cells (MDDC), and analyzed for their ability to proliferate (CFSElow) and produce cytokines. b Shown is the frequency of T-cells proliferating in response to C. albicans hyphae, CMV, or SEB at day 5 post co-culture. c Shown is the frequency of cytokine-expressing T-cells proliferating in response to C. albicans within each subset. d–f C. albicans-specific T-cells were further analyzed for the co-expression of IL-17A with IFN-γ or TNF-α. d Results are from one donor representative of results obtained with matched subsets from four different donors. (e–f) Shown is the frequency and MFI of C. albicans-specific T-cells expressing IL-17A either alone (IL-17A+IFN-γ−) (e) or in combination with IFN-γ (IL-17A+IFN-γ+) (f). b–f The positivity gates were defined based on FMO controls. b, c and e, f Shown are results (mean ± SEM) on matched samples from n = 4 different. Paired t-Test p-values are indicated in the figures. ND not determined