HIV-1 capsid uncoating initiates after the first strand transfer of reverse transcription

Retrovirology

Table 1 Real-time PCR primers and probes

Early: Strong stop cDNA (-sscDNA) (137 bases)^a
Forward primer	oHC64	5′-TAACTAGGGAACCCACTGC
Reverse primer	oHC65	5′-GCTAGAGATTTTCCACACTG
Probe	oHC66	5′-FAM-ACACAACAGACGGGCACACACTA-TAMRA

EGFP (1426 bases)^a
Forward primer	EGFP.F	5′-GCGCACCATCTTCTTCAAGG
Reverse primer	EGFP.R	5′-GTGTCGCCCTCGAACTTCAC
Probe	EGFP.Probe	5′-FAM-CGGCAACTACAAGACCCGCGC-TAMRA

Late: Second strand transfer ((+)strand) (3958 bases)^a
Forward primer	oHC64	5′-TAACTAGGGAACCCACTGC
Reverse primer	GagM661as	5′-CTGCGTCGAGAGAGCTCCTCTGGTT
Probe	oHC66	5′-FAM-ACACAACAGACGGGCACACACTA-TAMRA

^aThe expected length of the corresponding reverse transcribed DNA is indicated in brackets

ISSN: 1742-4690