Fig. 7

Identification of the G-specific amino acids required to restore the functionality in B 202G-V3C Env. a Schematic representation of the Env constructed by inserting in the V3C sequence, amino acids from the G isolate, according to the scheme. Color code: Env B grey, Env G black. b Functionality of the Env drawn in a (numbering of the samples is according to the numbering provided on the right of the drawings, in a). The value of infectivity for B 202G-V3C Env is given as reference on the left of the dotted line and the stars indicate that the functionality of the various Env increases significantly with respect to this reference. One star: p < 0.05; two stars, p < 0.01. Percentages of functionality were calculated as for Fig. 1. n varies between 3 and 5, depending on the sample considered. c Schematic drawing (left) and functionality (right) of B 202G/328G (the functionality of B 202G is given as reference on the left of the dotted line)