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Table 1 Main results of immunophenotypical and virological reservoir analysis of patients enrolled in the study according to their CD4+ cells count at the time of cART initiation

From: Major influence of CD4 count at the initiation of cART on viral and immunological reservoir constitution in HIV-1 infected patients

  Blood CD4+ T count at the time of diagnosis (cells/mm3) p value
>350 “high-level CD4 group” (n = 9) <350 “low-level CD4 group” (n = 15)
Immunophenotypical analysis of T cells
 MFI CD3+/PD1+ T cells
  Blood 17.8 [16.9–28] 17.7 [16.5–22] 0.79
  Rectum 30.9 [23.9–52.2] 38.8 [27.5–44.6] 0.88
 p24+/CD4+ T cells (% of CD4+ T cells)
  Blood 0.71 [0.26–1.45] 0.92 [0.39–1.59] 0.55
  Rectum 8.62 [2.97–16.72] 7.23 [2.42–27.46] 0.96
 MFI CXCR4 in CD4+ T cells
  Blood 17.78 [15.03–24] 17.33 [16.69–21.74] 0.72
  Rectum 17.44 [15.42–19.13] 16.15 [16.02–18.61] 0.46
 MFI CCR5 in CD4+ T cells
  Blood 42.03 [37.82–46.63] 40.75 [38.47–46.23] 0.97
  Rectum 51.01 [43.41–65.4] 52.64 [45.02–74.96] 1
 Th17 cells/Treg ratio
  Blood 2.9 [1.4–4.3] 2.2 [1.5–3] 0.64
  Rectum 1.4 [1.1–2.8] 1.3 [1.1–2.5] 0.96
 Blood/rectum ratio of β7+ expression in CD4+ T cells 0.9 [0.8–1.2] 1.6 [1.3–1.8] 0.0006
 Blood/rectum ratio of β7+ expression in CD8+ T cells 0.7 [0.5–1.2] 0.8 [0.7–1] 0.54
 MFI CCR9 in CD4+ T cells
  Blood 9.67 [9.29–10.65] 10.13 [9.27–11.25] 0.6
  Rectum 18.19 [13.93–23.51] 20.02 [16.05–25.23] 0.6
 MFI CCR9 in CD8+ T cells
  Blood 14.03 [13.01–20.02] 13.73 [13.19–17.29] 0.82
  Rectum 19.11 [16.41–24.14] 16.36 [14.78–18.78] 0.12
 Naive CD4+ T cells (% of CD4+ T cells)
  Blood 33.48 [27.55–46] 28.63 [17.03–42.8] 0.36
  Rectum 5.07 [1.96–10.35] 4.99 [3.54–7.12] 0.73
 CM CD4+ T cells (% of CD4+ T cells)
  Blood 38.29 [31.37–44.25] 37.06 [26.74–48.41] 0.97
  Rectum 24.38 [21.39–41.73] 15.42 [12.75–41.48] 0.39
 EM CD4+ T cells (% of CD4+ T cells)
  Blood 23.83 [11.89–27.31] 22.59 [12.78–33.74] 0.55
  Rectum 66.27 [53.45–71.44] 67.99 [50.44–82.32] 0.49
 EMRA CD4+ T cells (% of CD4+ T cells)
  Blood 4.83 [1.73–8.08] 2.23 [0.55–4.53] 0.22
  Rectum 0.61 [0.45–1.77] 0.39 [0.12–5.2] 0.66
Immunophenotypical analysis of B cells
 Blood naive IgD+ B cells (% of conventional B cells) 76.6 [60.6–79.1] 82.2 [77.3–87.6] 0.05
 Blood activated IgD B cells (% of conventional B cells) 23.1 [20.8–38.8] 17.6 [12.4–22.4] 0.05
 Memory B cells (% of conventional B cells) 5.94 [1.68–18.05] 4.05 [2.07–5.66] 0.34
Virological reservoir analysis
 HIV DNA load (log10 copies/millions of cells)
  Blood 2.41 [2.03–2.93] 3.48 [3.17–3.7] 0.01
  Rectum 3.06 [1.93–3.63] 3.6 [3.4–4.08] 0.04
  1. Medians of MFI or percentages [range]
  2. CM central memory, EM effector memory, EMRA effector memory T cells expressing RA