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Table 1 Main results of immunophenotypical and virological reservoir analysis of patients enrolled in the study according to their CD4+ cells count at the time of cART initiation

From: Major influence of CD4 count at the initiation of cART on viral and immunological reservoir constitution in HIV-1 infected patients

 

Blood CD4+ T count at the time of diagnosis (cells/mm3)

p value

>350 “high-level CD4 group” (n = 9)

<350 “low-level CD4 group” (n = 15)

Immunophenotypical analysis of T cells

 MFI CD3+/PD1+ T cells

  Blood

17.8 [16.9–28]

17.7 [16.5–22]

0.79

  Rectum

30.9 [23.9–52.2]

38.8 [27.5–44.6]

0.88

 p24+/CD4+ T cells (% of CD4+ T cells)

  Blood

0.71 [0.26–1.45]

0.92 [0.39–1.59]

0.55

  Rectum

8.62 [2.97–16.72]

7.23 [2.42–27.46]

0.96

 MFI CXCR4 in CD4+ T cells

  Blood

17.78 [15.03–24]

17.33 [16.69–21.74]

0.72

  Rectum

17.44 [15.42–19.13]

16.15 [16.02–18.61]

0.46

 MFI CCR5 in CD4+ T cells

  Blood

42.03 [37.82–46.63]

40.75 [38.47–46.23]

0.97

  Rectum

51.01 [43.41–65.4]

52.64 [45.02–74.96]

1

 Th17 cells/Treg ratio

  Blood

2.9 [1.4–4.3]

2.2 [1.5–3]

0.64

  Rectum

1.4 [1.1–2.8]

1.3 [1.1–2.5]

0.96

 Blood/rectum ratio of β7+ expression in CD4+ T cells

0.9 [0.8–1.2]

1.6 [1.3–1.8]

0.0006

 Blood/rectum ratio of β7+ expression in CD8+ T cells

0.7 [0.5–1.2]

0.8 [0.7–1]

0.54

 MFI CCR9 in CD4+ T cells

  Blood

9.67 [9.29–10.65]

10.13 [9.27–11.25]

0.6

  Rectum

18.19 [13.93–23.51]

20.02 [16.05–25.23]

0.6

 MFI CCR9 in CD8+ T cells

  Blood

14.03 [13.01–20.02]

13.73 [13.19–17.29]

0.82

  Rectum

19.11 [16.41–24.14]

16.36 [14.78–18.78]

0.12

 Naive CD4+ T cells (% of CD4+ T cells)

  Blood

33.48 [27.55–46]

28.63 [17.03–42.8]

0.36

  Rectum

5.07 [1.96–10.35]

4.99 [3.54–7.12]

0.73

 CM CD4+ T cells (% of CD4+ T cells)

  Blood

38.29 [31.37–44.25]

37.06 [26.74–48.41]

0.97

  Rectum

24.38 [21.39–41.73]

15.42 [12.75–41.48]

0.39

 EM CD4+ T cells (% of CD4+ T cells)

  Blood

23.83 [11.89–27.31]

22.59 [12.78–33.74]

0.55

  Rectum

66.27 [53.45–71.44]

67.99 [50.44–82.32]

0.49

 EMRA CD4+ T cells (% of CD4+ T cells)

  Blood

4.83 [1.73–8.08]

2.23 [0.55–4.53]

0.22

  Rectum

0.61 [0.45–1.77]

0.39 [0.12–5.2]

0.66

Immunophenotypical analysis of B cells

 Blood naive IgD+ B cells (% of conventional B cells)

76.6 [60.6–79.1]

82.2 [77.3–87.6]

0.05

 Blood activated IgD B cells (% of conventional B cells)

23.1 [20.8–38.8]

17.6 [12.4–22.4]

0.05

 Memory B cells (% of conventional B cells)

5.94 [1.68–18.05]

4.05 [2.07–5.66]

0.34

Virological reservoir analysis

 HIV DNA load (log10 copies/millions of cells)

  Blood

2.41 [2.03–2.93]

3.48 [3.17–3.7]

0.01

  Rectum

3.06 [1.93–3.63]

3.6 [3.4–4.08]

0.04

  1. Medians of MFI or percentages [range]
  2. CM central memory, EM effector memory, EMRA effector memory T cells expressing RA