Fig. 3
Repartition of naive and activated B cells in blood, according to the CD4+ T cells at the initiation of cART. Frequency of IgD+ naive B cells (a) in blood of HIV-1 infected individuals, treated above or below 350 CD4+/mm3 T cells count. Horizontal lines represent median values and ranges. Correlation between current CD4+/CD8+ T cells ratio in blood and percentage of IgD+ naive B cells in blood of HIV infected individuals (b). Each symbol represents an individual. Flow cytometry analysis of IgD+/CD19+/CD5− B cells in the blood of two HIV-infected individual representatives of “high” and “low-level CD4 group” respectively (c). Gating strategy: lymphoid cells were gated using the FSC/SSC parameters. Then, B cells were gated by their expression of CD19 and were separated in CD5+ non-conventional and CD5− conventional B cells. IgD expression was studied in CD5− conventional B cells to plot naive and activated B cells