Fig. 2
Inhibition of dM infection by dNK cells added at different times. a dMs were infected with HIV-1BaL at an MOI of 10−3. dNK cells were added to dMs before infection, 1, 3 or 16 h after infection, at a ratio 1 dM:5 dNK. For each donor, all conditions were performed. Viral production was followed by the quantification of the p24 Ag in the supernatants. The percentage of inhibition of the p24 Ag production was calculated at day 20 post-infection. Each symbol represents one individual donor. The medians of 6 donors are displayed on the graph. The sign-rank test for paired data was used. § p = 0.031 between the different coculture conditions (dNK cells added to dMs before, 1, 3 or 16 h after infection). *p = 0.031 between dMs alone and in coculture with dNK cells. b The viability of dNK cells added to dMs just after (black) or 16 h after dM infection (green) was analysed by flow cytometry, with a viability dye labelling the dead cells. A representative FACS histogram gated on CD56+ cells is shown. The expression of CD8, CD69, CD85j, NKG2D, NKp46, NKp44 and NKp30 were analysed on dNK cells just after or 16 h after dM infection by flow cytometry staining. Flow cytometry graphs gated on CD56+ cells from a representative sample are displayed