Fig. 2

Identification of miRNAs tightly bound by Tat complex. HEK 293T cells were transfected with Flag Tat plasmid and Tat complexes were isolated 48 h post-transfection using anti-Flag IP. a RNA from Tat complexes and whole cells was used as input into a polyA mediated RT-qPCR array capable of detecting over 300 human miRNAs. Shown is the fold enrichment in the Tat complex of the 68 miRNAs found to bind to Tat. The miRNAs indicated by asterisk show a twofold enrichment in the Tat complex as compared to the whole cell in all replicates. b Corresponding levels of miRNA in the whole cell RNA fraction