Fig. 1From: The inhibition of microRNAs by HIV-1 Tat suppresses beta catenin activity in astrocytesTat association with cellular miRNA. HEK 293T cells were transfected with Flag Tat-WT, K51A or K41A. Forty-eight hours post-transfection Tat complexes were isolated by anti-Flag IP and RNA was extracted from these complexes or whole cells. RT-qPCR was used to determine if target miRNA associated with Tat complexBack to article page