Fig. 1
From: Mechanisms of escape from the PGT128 family of anti-HIV broadly neutralizing antibodies
CDRH3 interaction with V3 is critical for neutralization by PGT128 and PGT130. a Residues in the 323IGDIR327 motif of JR-FL were mutated to proline using site-directed mutagenesis and impact on neutralization by PGT128, PGT130 and PGV04 determined. Mutations G324P and R327P produced non-infectious virus. V3 contact residues in the CDRH3 of b PGT128 and c PGT130 were reverted to germ line or mutated to proline using site-directed mutagenesis and impact on neutralization of JR-FL and JR-CSF determined. Data is reported as fold change in IC50 compared to WT (Fold change = IC50 mutant/IC50 WT) and colour coded according to the figure key