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Fig. 2 | Retrovirology

Fig. 2

From: ATP generation in a host cell in early-phase infection is increased by upregulation of cytochrome c oxidase activity via the p2 peptide from human immunodeficiency virus type 1 Gag

Fig. 2

HIV-1 p2 peptide interactd with mitochondrial cytochrome c oxidase. a For cotransformation assay using the positive clones from the initial Y2H screening, the bait plasmid (pGBKT7-p2) and prey plasmid encoding the mitochondrial intermembrane space domain (N214–F235) of MT-CO1 (pGADT7-MT-CO1) were cotransformed into Y2Hgold to confirm the interactions in yeast and grown on DDO/X and QDO/X/A. Growth on QDO/X/A indicated interaction between the p2 peptide and MT-CO1. b P2 mutants (E2A, M4A, S5A, and Q6A) were used to investigate their interactions with MT-CO1. The mitochondrial intermembrane space domain (N214–F235) of MT-CO1 interacted with the mutant E2A, M4A, and S5A p2 peptides, but not with the Q6A mutant p2 peptide

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Retrovirology

ISSN: 1742-4690

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