Fig. 2

Imaging MLV Entry into mitotic cells. a U/R/EMTB-mCherry cells, arrested at mitosis by nocodazole, were infected with DiD-labeled wt GFP virus and visualized 75 min postinfection. Viral cores and viral membranes are in green and red, respectively. Cellular EMTB fluorescence is pseudocolored in blue. Empty arrowheads mark intracellular GFP⁺DiD⁺ puncta. Asterisks mark intracellular GFP⁺DiD⁻ puncta, overlapping dark regions, likely representing mitotic chromosomes. Nocodazole-arrested (b) or interphase (c) U/R/H2A-RFP cells, whose cellular memebranes were labeled with DiD (red), were infected with wt GFP virus (green) and visualized 1 h postinfection. Full arrowheads mark peripheral/extracellular cores that do not overlap the DiD staining. Empty arrowheads mark intracellular viral cores overlapping dense DiD staining. Asterisks mark viral cores attached to mitotic chromosomes, pseudocolored in blue. Scale bars 10 µm