Fig. 5

Kinetics of RNA plasma viral load and mRNA levels of BST2 and MX1 in SIV-infected macaques. Plasma viral load as well as relative BST2 and MX1 mRNA levels in PBMC were determined longitudinally in seven macaques before and after inoculation with SIVmac251 MPBMC (a–c). Viral load is depicted as log-transformed RNA copies per millilitre (ml) plasma (a). Relative BST2 mRNA (b) and relative MX1 mRNA (c) in PBMC were calculated as copy numbers per 100 copies of GAPDH. Data are expressed as fold increase over baseline after normalization to the mean of three pre-infection values. Fine grey lines with symbols represent individual infected animals. Fine black lines with closed triangles depict the one animal inoculated but not infected. Bold lines show mean values of infected animals. p value show a significant difference to the mean of the three pre-infection values calculated by Mann–Whitney’s U test. d Relationship between relative BST2 and MX1 mRNA levels in PBMC from rhesus macaques during acute SIV infection (10–14 dpi). Line shows linear regression; r, Spearman’s correlation coefficient; p, p value. Samples from LTNPs (filled triangles) and progressor (open circles) rhesus macaques were analysed for viral load (e), relative BST2 (f) and relative MX1 (g) RNA levels at 2 wpi. h Relationship between pre-infection BST2 mRNA levels and viral load at 2 wpi. Each data point represents one individual animal. Horizontal lines within the clusters are depicting the median. Group comparisons were calculated using Mann–Whitney’s U test