Fig. 3

Single VSV-G pseudovirus fusion results in loss of GFP-Vpr from viral core. VSVpp co-labeled with Gag-imCherry (content marker, red) and GFP-Vpr (core marker, green) were pre-bound to CV-1 cells and allowed to fuse at 37 °C in Fluorobrite DMEM medium under 5 % CO₂. a Images of mCherry release from single virus followed by gradual loss of the GFP-Vpr signal. b Dark red and green traces show sum fluorescence of mCherry and GFP channels, respectively, obtain by tracking the virus shown in a. For comparison, fluorescence intensities of mCherry and GFP for a non-fusing particle are shown (beige and cyan traces, respectively). c Single virus tracking results of another fusing VSVpp. Occasional spikes in fluorescence (for example, at the 38 min time point) are due to a transient overlap of the particle of interest with either another particle or with cell’s autofluorescent features