Fig. 6
From: Conserved determinants of lentiviral genome dimerization
DIS mutations affect retroviral 5′-leader dimerization. a Structure of the native SIVcpzTAN1 DIS stem-loop within the intact leader and mutations (red) that extend its native DIS palindrome. b Native agarose gel electrophoresis data obtained for SIVcpzTAN1 wild-type (5′-LWT) and mutant leader RNAs (5′-LGC and 5′-LHIV-1 loop) after 1-h incubation in PI buffer and measured using TB and TBM running buffers. c Structure of the native HIV-2ROD DIS stem-loop within the intact leader and mutations (red) that obstructs the DIS palindrome (5′-LCCA) or enhances DIS intermolecular loop–loop interactions (5′-LHIV-1 loop). d Native gel data also obtained for HIV-2ROD wild-type and mutant leader RNAs. DIS mutations with extended/enhanced DIS palindromes favor the dimer formation, whereas mutation with obstructed DIS palindrome favors the monomer formation