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Table 3 Selection of proteins deregulated at least ±2.0-fold in Jurkat-Tat101 versus control cells

From: Intracellular expression of Tat alters mitochondrial functions in T cells: a potential mechanism to understand mitochondrial damage during HIV-1 replication

Gene Protein Tat72 vs TetOff Tat101 vs TetOff Biological process Peptide sequence X-corr
CORO1A Coronin-1A 9.3 36.8 Actin cytoskeleton organization KLQATVQELQK 2.93
MYL12B Myosin regulatory light chain 12B 3.0 13.2 Regulation of cell shape ELLTTMGDRFTDEEVDELYR
GNFNYIEFTR
1.75
2.60
EZR Ezrin 11.2 11.9 Actin filament bundle assembly QLLTLSSELSQAR
QLLTLSSELSQARDENKR
1.79
2.06
SEPT2 Septin-2 0.0 5.2 Actin cytoskeleton organization TMLITHMQDLQEVTQDLHYENFR 2.29
CFL1 Cofilin-1 0.9 5.2 small GTPase signal transduction LGGSAVISLEGKPL 2.33
ACTN4 Actinin-alpha-4 0.0 4.4 Actin filament bundle assembly MAPYQGPDAVPGALDYK
GISQEQMQEFR
2.11
2.11
TUBB2C Tubulin beta-2C 6.4 4.4 Microtubule organization INVYYNEATGGK 1.75
SLC9A3R1 Na/H exchange regulatory cofactor (NHE-RF1) 3.1 3.5 Actin cytoskeleton organization SVDPDSPAEASGLR
SVDPDSPAEASGLRAQDR
1.95
1.33
PPP1R12A Protein phosphatase 1 regulatory subunit 12A 5.2 2.8 Actin cytoskeleton organization NKETLIIEPEKNASR 2.12
TPM4 Tropomyosin alpha-4 chain 9.9 2.2 Actin cytoskeleton organization KIQALQQQADEAEDR 1.65
2.13
TBCA Tubulin-specific chaperone A 2.1 2.2 Tubulin assembly LVLDSVKLEA
RLEAAYLDLQR
1.78
1.84
VIM Vimentin 3.9 2.2 Cytoskeleton organization FADLSEAANR 1.75
GDI2 Rab GDP dissociation inhibitor beta 0.0 2.0 small GTPase signal transduction MTGSEFDFEEMKR 1.78
TUBA1B Tubulin alpha-1B 3.7 −3.4 Microtubule cytoskeleton organization TIGGGDDSFNTFFSETGAGK
DVNAAIATIK
1.91
2.00
TUBB Tubulin beta −1.3 −6.9 Microtubule cytoskeleton organization FWEVISDEHGIDPTGTYHGDSDLQLDR
AILVDLEPGTMDSVR
EVDEQMLNVQNK
MSMKEVDEQMLNVQNK
2.16
2.20
2.74
4.08
MYH10 Myosin-10 −2.3 −8.5 Actin filament-based movement ELDDATEANEGLSREVSTLKNR
QLEEAEEEATRANASR
ELDDATEANEGLSR
1.99
2.38
2.78
ARHGDIB Rho GDP-dissociation inhibitor 2 −2.6 −10.0 small GTPase signal transduction TLLGDGPVVTDPKAPNVVVTR 1.97
STMN1 Stathmin −28.5 −30.5 Microtubule depolymerization RAsGQAFELILsPR 2.19
TMSL3 Thymosin beta-4-like protein 3 −28.8 −33.4 Actin cytoskeleton organization NPLPSKETIEQEKQAGES
NPLPSKETIEQEK
TETQEKNPLPSKETIEQEK
1.80
2.31
2.65
ACTB Actin cytoplasmic −15.0 −43.7 Actin cytoskeleton organization DSYVGDEAQSK
DLYANTVLSGGTTMYPGIADR
DSYVGDEAQSKR
HQGVMVGMGQK
AVFPSIVGRPR
TTGIVMDSGDGVTHTVPIYEGYALPHAI
QEYDESGPSIVHR
HQGVMVGMGQKDSYVGDEAQSK
IWHHTFYNELR
MQKEITALAPSTMK
1.83
1.87
1.88
1.89
1.98
2.15
2.37
2.81
3.31
3.52
  1. Only proteins related to actin and tubulin cytoskeleton networks were selected. All peptides selected showed 95 % probability for protein expression and a minimum X-corr of 1.75