Table 3 Selection of proteins deregulated at least ±2.0-fold in Jurkat-Tat101 versus control cells
Gene | Protein | Tat72 vs TetOff | Tat101 vs TetOff | Biological process | Peptide sequence | X-corr |
|---|---|---|---|---|---|---|
CORO1A | Coronin-1A | 9.3 | 36.8 | Actin cytoskeleton organization | KLQATVQELQK | 2.93 |
MYL12B | Myosin regulatory light chain 12B | 3.0 | 13.2 | Regulation of cell shape | ELLTTMGDRFTDEEVDELYR GNFNYIEFTR | 1.75 2.60 |
EZR | Ezrin | 11.2 | 11.9 | Actin filament bundle assembly | QLLTLSSELSQAR QLLTLSSELSQARDENKR | 1.79 2.06 |
SEPT2 | Septin-2 | 0.0 | 5.2 | Actin cytoskeleton organization | TMLITHMQDLQEVTQDLHYENFR | 2.29 |
CFL1 | Cofilin-1 | 0.9 | 5.2 | small GTPase signal transduction | LGGSAVISLEGKPL | 2.33 |
ACTN4 | Actinin-alpha-4 | 0.0 | 4.4 | Actin filament bundle assembly | MAPYQGPDAVPGALDYK GISQEQMQEFR | 2.11 2.11 |
TUBB2C | Tubulin beta-2C | 6.4 | 4.4 | Microtubule organization | INVYYNEATGGK | 1.75 |
SLC9A3R1 | Na/H exchange regulatory cofactor (NHE-RF1) | 3.1 | 3.5 | Actin cytoskeleton organization | SVDPDSPAEASGLR SVDPDSPAEASGLRAQDR | 1.95 1.33 |
PPP1R12A | Protein phosphatase 1 regulatory subunit 12A | 5.2 | 2.8 | Actin cytoskeleton organization | NKETLIIEPEKNASR | 2.12 |
TPM4 | Tropomyosin alpha-4 chain | 9.9 | 2.2 | Actin cytoskeleton organization | KIQALQQQADEAEDR | 1.65 2.13 |
TBCA | Tubulin-specific chaperone A | 2.1 | 2.2 | Tubulin assembly | LVLDSVKLEA RLEAAYLDLQR | 1.78 1.84 |
VIM | Vimentin | 3.9 | 2.2 | Cytoskeleton organization | FADLSEAANR | 1.75 |
GDI2 | Rab GDP dissociation inhibitor beta | 0.0 | 2.0 | small GTPase signal transduction | MTGSEFDFEEMKR | 1.78 |
TUBA1B | Tubulin alpha-1B | 3.7 | −3.4 | Microtubule cytoskeleton organization | TIGGGDDSFNTFFSETGAGK DVNAAIATIK | 1.91 2.00 |
TUBB | Tubulin beta | −1.3 | −6.9 | Microtubule cytoskeleton organization | FWEVISDEHGIDPTGTYHGDSDLQLDR AILVDLEPGTMDSVR EVDEQMLNVQNK MSMKEVDEQMLNVQNK | 2.16 2.20 2.74 4.08 |
MYH10 | Myosin-10 | −2.3 | −8.5 | Actin filament-based movement | ELDDATEANEGLSREVSTLKNR QLEEAEEEATRANASR ELDDATEANEGLSR | 1.99 2.38 2.78 |
ARHGDIB | Rho GDP-dissociation inhibitor 2 | −2.6 | −10.0 | small GTPase signal transduction | TLLGDGPVVTDPKAPNVVVTR | 1.97 |
STMN1 | Stathmin | −28.5 | −30.5 | Microtubule depolymerization | RAsGQAFELILsPR | 2.19 |
TMSL3 | Thymosin beta-4-like protein 3 | −28.8 | −33.4 | Actin cytoskeleton organization | NPLPSKETIEQEKQAGES NPLPSKETIEQEK TETQEKNPLPSKETIEQEK | 1.80 2.31 2.65 |
ACTB | Actin cytoplasmic | −15.0 | −43.7 | Actin cytoskeleton organization | DSYVGDEAQSK DLYANTVLSGGTTMYPGIADR DSYVGDEAQSKR HQGVMVGMGQK AVFPSIVGRPR TTGIVMDSGDGVTHTVPIYEGYALPHAI QEYDESGPSIVHR HQGVMVGMGQKDSYVGDEAQSK IWHHTFYNELR MQKEITALAPSTMK | 1.83 1.87 1.88 1.89 1.98 2.15 2.37 2.81 3.31 3.52 |