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Table 1 Sequences targeted by the shRNA expression plasmids used

From: Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production

shRNA

Target sequence

shControl

TGGTTTACATGTCGACTAA

shEAP20-1

CGTCAAGCTACAGCGAAAG

shEAP20-2

CCTCGAGTGGTTGGATAAG

shEAP30-6

GGAACTACATCAACAGGTG

shEAP30-7

CCAGGATGTCAGTCAAGAT

shEAP30-8

ACCTGATCAGAGCCATCAA

shEAP45-1

GAATAAGGGCTGTAGGAAT

shEAP45-2

TGATCAAGGCTAAGGAAAT

  1. The target sequence for shControl was derived from Dharmacon ON-TARGETplus siRNA. The remaining were selected using Clontech RNAi Target Sequence Selector (http://bioinfo.clontech.com/rnaidesigner/sirnaSequenceDesignInit.do) and MWG Biotech websites (http://www.eurofinsdna.com/products-services/sirna/sirna-design.html).
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Retrovirology

ISSN: 1742-4690

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