Fig. 2
From: Determinants in HIV-2 Env and tetherin required for functional interaction
The HIV-2 Env ectodomain is not sufficient to overcome tetherin. a HIV-1 VLPs were produced in the presence of the WT ROD10 Env, or increasing amounts of ROD10 Envgpi (2, 4, 8 μg). VLP release was analyzed by Western blotting and results normalized to the no tetherin control, for n = 3 independent experiments, p < 0.05 (*). b Cell surface expression of ROD10 Envgpi was confirmed by flow cytometry. The light grey line represents cells stained with secondary antibody only, the dark line represents the indicated ROD10 Env variant. Mean fluorescence intensity (MFI) for each panel is shown. c 293T cells were co-transfected with tetherin or GFP-tagged tetherin, together with ROD10 Env or ROD10 Envgpi at the indicated amounts. Cells were lysed 24 h later and GFP-tagged proteins were immunoprecipitated (IP) with anti-GFP MicroBeads, followed by Western blotting of the input lysates (1%) and IP products using anti-Env or anti-tetherin antibodies.