Figure 3
From: Human T-cell leukemia virus type-I Tax induces the expression of CD83 on T cells
Western blot analysis of CD83 expressed in the HTLV-I+ T cell line HUT102. Cell lysates from control 293T/CT (lanes 1 and 5), CD83-transfected 293/TCD83 (lanes 2 and 6), CEM (lanes 3 and 7), and HUT102 (lanes 4 and 8) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 5–20% gel, and blotted onto PVDF membranes. The membranes were incubated with either rat anti-human CD83 monoclonal antibody (mAb) (clone W#83-8, lanes 1–4) or isotype control rat IgG (clone WAP-24, lanes 5–8) antibody, followed by treatment with HRP-labeled goat anti-rat IgG antibody.