Figure 3

MVC resistance and viral replicative capacity: The effects of amino acid changes in the MVC-Sens and MVC-Res V3 loops and dependence on the cell type. U87-CD4/CCR5 cells (a, b), PBMCs (c, d) or CD4- and CCR5-expressing HEK 293T cells (e, f) were infected with equal amounts (10 ng of Gag p24) of MVC-Sens, MVC-Res or their related variants S(V3R), R(V3S), S(P/S), S(+A), R(S/P) or R(−A), in the absence or in the presence of 10 μM MVC. The percents of infection inhibition (panels a, c, e) were determined as indicated in the legend of Figure 2. Viral infectivities (b, d, f) were determined by measuring luciferase activity in the cell lysates 30 h (U87, HEK) or 48 h (PBMCs) post-infection and are expressed as percent infectivity relative to that of MVC-Sens (100%). Results are mean ± SEM of 3–8 independent experiments performed in triplicate. ***P < 0.001 in unpaired two-tailed Student t test.