Figure 6

Suboptimal correction and fixation of the mutation in the primer binding site (PBS) over time. SupT1-CCR5 cells were infected with SIVmac239WT (a) or with a nonreplicating SIVmac239WT∆Env which was pseudotyped with SIVmac239WT Env to produce a single round infection model (b). Samples were collected at 12 and 24 h and then daily. The PBS of the integrated genome was sequenced by SGA. For both panels, the percent of each variant per day is plotted and a linear trend line was added for each population. The percentage of sequences bearing the suboptimal thymine/adenine (T/A) base is shown in red, those bearing the optimal cytosine/guanine (C/G) base is shown in green, and those containing the mismatched cytosine/adenine (C/A) base is shown in blue.