Fig. 5
(a) SHAPE reactivities of the in vitro transcribed tRNA are shown on the secondary structure model of tRNALys3. Boxed nucleotides are implicated in annealing to viral RNA. SHAPE reactivities of tRNALys3 nts involved in annealing are shown in (b) for the anti-poly(a) region, and in (c) for the anti C-rich region. Reactivities from unannealed in vitro transcribed tRNA, annealed tRNA from wild type and Pr- virions are shown in white, grey and black respectively. The results shown are the averages of three readings at each nucleotide position. The errors correspond to the standard deviations calculated at each nucleotide position