Figure 6

MMTV infection is not mediated through human transferrin receptor 1. (A) CHO cells transfected with mTfR1 or hTfR1expression plasmids were transduced with MMTV-based vector particles carrying the wild-type (WT) or mutant (G42E) MMTV(C3H) Env, or MMTV cores pseudotyped with clade B arenavirus GPs (Junin virus, JUNV; Tacaribe virus, TCRV). Expression of hTfR1 was confirmed by western blotting (inset); mTfR1 was not recognised by the α-TfR1 antibody (ab137944, Abcam). (B) Hs578T cells transfected with hTFR1-specific siRNA or non-targeting siRNA were transduced with MMTV-based vector particles carrying the wild-type (WT) or mutant (G42E) MMTV(C3H) Env, or MMTV cores pseudotyped with clade B arenavirus GPs. hTfR1 knock-down was confirmed by western blotting (inset). (A & B) Transduction of target cells was detected by FCM 3 days post infection. Transduction levels were made either relative to the value obtained for control siRNA (A) or relative to the value obtained for the mock-transfected cells (B) and expressed as percentage of those controls. The values shown are mean ± standard errors from three independent experiments.