Figure 6
From: Key determinants of target DNA recognition by retroviral intasomes
BRD4 protein and interaction with Rev-A IN. (A) Schematic of human BRD4 isoform C (NCBI reference sequence NP_055114.1) highlighting various protein domains and the 426–720 fragment used in this study. (B) Ni-NTA pull-down of purified BRD4462–720 by His6-tagged Rev-A IN. Migration positions of standards (in kDa) are labeled to the left. Lanes 1–4: 20% reaction input of the indicated proteins. Lanes 5 and 6: the indicated BRD4462–720 protein was incubated with Ni-NTA beads in the absence of Rev-A IN. Lanes 7 and 8: the indicated BRD4462–720 protein was incubated with Rev-A IN-bound beads. The gel is representative of results obtained from three independent experiments.