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Table 2 Primers used for semi-quantitative and quantitative RT-PCR

From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication

Target RNA Primer Sequence
Viral mRNA classes #1544 (E1) 5′ CTT GAA AGC GAA AGT AAA GC 3′
  #3392 (E7) 5′ CGT CCC AGA TAA GTG CTA AGG 3′
  #640 (I4) 5′ CAA TAC TAC TTC TTG TGG GTT GG 3′
  #3632 (E4) 5′ TGG ATG CTT CCA GGG CTC 3′
  #3637 (E5) 5′ GAG AAG CTT GAT GAG TCT GAC 3′
Tat1 #3631 5′ CGG CGA CTG AAT TGG GTG T 3′
  #3632 5′ TGG ATG CTT CCA GGG CTC 3′
All viral RNAs #3387 5′ TTG CTC AAT GCC ACA GCC AT 3′
  #3388 5′ TTT GAC CAC TTG CCA CCC AT 3′
GAPDH #5163 5′ CCA CTC CTC CAC CTT TGA 3′
  #5164 5′ ACC CTG TTG CTG TAG CCA 3′