Table 2 Primers used for semi-quantitative and quantitative RT-PCR
From: Balanced splicing at the Tat-specific HIV-1 3′ss A3 is critical for HIV-1 replication
Target RNA | Primer | Sequence |
|---|---|---|
Viral mRNA classes | #1544 (E1) | 5′ CTT GAA AGC GAA AGT AAA GC 3′ |
#3392 (E7) | 5′ CGT CCC AGA TAA GTG CTA AGG 3′ | |
#640 (I4) | 5′ CAA TAC TAC TTC TTG TGG GTT GG 3′ | |
#3632 (E4) | 5′ TGG ATG CTT CCA GGG CTC 3′ | |
#3637 (E5) | 5′ GAG AAG CTT GAT GAG TCT GAC 3′ | |
Tat1 | #3631 | 5′ CGG CGA CTG AAT TGG GTG T 3′ |
#3632 | 5′ TGG ATG CTT CCA GGG CTC 3′ | |
All viral RNAs | #3387 | 5′ TTG CTC AAT GCC ACA GCC AT 3′ |
#3388 | 5′ TTT GAC CAC TTG CCA CCC AT 3′ | |
GAPDH | #5163 | 5′ CCA CTC CTC CAC CTT TGA 3′ |
#5164 | 5′ ACC CTG TTG CTG TAG CCA 3′ |