Effect of CCL2 neutralization on Mx2 expression. MDM were treated with anti-CCL2 or control Ab (2.5 μg/ml) or IFN-α (1000 U/ml) for 20 h. (A) Total RNA was extracted, retrotranscribed, amplified and the 2ΔΔCt values for Mx2 transcripts were calculated as described in Materials and Methods. The result from a single representative experiment of 3 independently performed is shown. (B) Cells were lysed and Mx2 protein expression in whole cell extracts was detected by western blot. The result from a representative donor out of 6 analyzed is shown. (C) Densitometric analyses of Mx2 expression performed on immunoblotting of MDM extracts from the 6 different donors analyzed. The graph shows the ratio of Mx2 to actin OD determined by densitometry. **p < 0.01 (IFN-α vs. nil).