RC map for 57% of nucleotide positions in the pNL4-3 HIV-1 genome. The HIV-1 genome showing the log10 RC index for all 7,314 individual point mutations assessed for RC after library passage in CEM T-lymphocyte cell culture. HIV-1 genomic regions are identified. Color denotes nucleotide identity of base change: A: blue, C: yellow, G: red, and T: green. An inset high-resolution view of the 3’-LTR region (9442–9446) of the genome is also highlighted.