Figure 2
From: A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1
Interactions of DDX1, DDX3 and DDX17 with Nullbasic in vivo . HEK293T cells were transfected with either empty vector (pcDNA 3.1) or plasmid expressing NB-FLAG. The FLAG-tagged proteins and their binding partners were immunoprecipated with anti-FLAG beads. Total cell lystes and immunoprecipated proteins were separated by SDS-PAGE and target proteins were detected using anit-FLAG, anti-DDX1, anti-DDX3 and anti-DDX17 antibodies. The anti-CDK9 antibody was used to detect endogenous CDK9 as a positive control for NB interaction. The figure is representative of 3 independent experiments.