Genome-wide shRNA screening identifies host factors involved in early endocytic events for HIV-1-induced CD4 down-regulation

Table 1 Experimental setup overview for the validation of the hits obtained from the genome-wide shRNA screening

	Genome-wide screening	Validation 1	Validation 2
Cells	HeLa CD4++ cl1022	HeLa CD4++ cl1022	SupT1
Format	Pooled shRNA library	Single shRNA	Single shRNA
shRNA plasmid	pSIF H1 SBI (System Biosciences)	pLKO.1 (MISSION® Sigma Aldrich)	pLKO.1 (MISSION® Sigma Aldrich)
CD4 down-modulation inducer	LZRS-NA7 Nef-IRES-eGFP	LZRS-NA7 Nef-IRES-eGFP	HIV-1 NL4.3 eGFP

ISSN: 1742-4690