Figure 2

Results of validation 1: CD4 residual expression in HeLa CD4++ expressing Nef and the shRNAs targeting the selected 20 genes. Bar graph shows the residual CD4 expression in HeLa CD4++ expressing the shRNAs for the knock-down of the 20 selected hits and the Nef retroviral construct. The values are representative of two independent confirmation experiments and for the shRNA clone with most activity among the ones tested. The gray bar indicates the value obtained in cells transduced with the non-targeting scrambled shRNA control.