Figure 3
From: Re-visiting the functional Relevance of the highly conserved Serine 40 Residue within HIV-1 p6Gag
Effect of p6 S40 substitutions on viral infectivity. Viral particles were harvested from cell culture media of HEK 293 T cells transfected with proviral plasmids and tested for infectivity. Single round infectivity (A) was assessed by titration of viral particles on TZM-bl cells as described in the main text. Relative infectious titers on C8166 T-cells (B) were determined by endpoint titration. Values were normalized to p24 amounts determined by ELISA. Graphs show mean values and SD of one experiment using viral particles from six independent preparations. p-values in (A) and (B) were calculated using an unpaired student’s t-test (n.s. > 0.05, * < 0.05, *** < 0.001).