Figure 6
Amino acid sequences of mutants targeting the tip of V1 region and the base of V2 loop. (A) For a better localization of mutated amino acids, the sequence alignment between HIV-2ROD (GenBank accession number: M15390) and HIV-2MJC97 (GenBank accession number: EU021092) was included. The red boxes indicate the conserved regions between HIV-2ROD and HIV-2MJC97 amino acids sequences. (B) The tip of V1 of MJC97wt has the sequon ISTTDYSL (amino acids residues 113–120) that was changed to IPTDQEQE (MJC97mtV1) or PGSTLKPL (MJC97mtV1′). The MJC97mtV1V2 was obtained by replacing the sequon PTNET (amino acids residues 172–176) in the base of V2 loop of MJC97mtV1 by TNNES; MJC97V1V2′ was originated by changing the referred sequon of MJC97mtV1′ by PFNTT. Amino acids residues (in panel A and B) were numbered according to HIV-2ROD (GenBank accession number: M15390) or HIV-2MJC97 sequence (GenBank accession number: EU021092).