Figure 6

SHAPE and structural analyses of the SL4 domain of the pal II and PBS pal mutants. (A) SHAPE reactivity profile of the wild type and mutant RNAs. Blue boxes indicate pal II, ssPurines, and deletion/substitutions in pal II. Data are an average of at least three independent experiments. (B) Predicted structure of the wild type and mutant SL4 domain in the SHAPE-constrained RNA model by RNAstructure (C) The RNAstructure predicted site of interaction (point of dimerization) between two wild type gRNAs. The central 6 nt of pal II are shown in blue.