Figure 5

Relative infectivity of M-PMV CA-NTD mutants determined by single-round assay. HEK 293 T cells were co-transfected with WT or mutant pSARM-EGFP and pTMO vectors. At 48 h post-transfection, viruses were filtered from culture media and normalized by quantitative Western blotting for CA. Equivalent amounts of viruses were used to infect fresh HEK 293 T cells. At 48 h post-infection the cells were harvested and the numbers of GFP-positive cells were determined by flow cytometry (BD FACSaria). The mean percentage of five independent infectivity measurements (with calculated standard deviations) for each mutant relative to the WT is shown.