Figure 7
From: ADAR1 enhances HTLV-1 and HTLV-2 replication through inhibition of PKR activity
ADAR1 expression reverses IFN- α inhibitory effect on HTLV-1 and HTLV-2. 293--T cells were transfected with 4 μg of (A) pACH or (B) pH6neo molecular clone. When required, 500 ng of an ADAR1 expression plasmid were also transfected and cells were treated with increasing amount of IFN-α (0-1000 U/mL) for 48 hours. Western blot analyses were performed on 60 μg of proteins obtained from whole cell extracts using anti-ADAR1, anti-p24gag, anti-Tax1, anti-Tax2, anti-PKR, anti-phospho-PKR and anti-actin antibodies.