Figure 2

ADAR1 has a proviral effect on de novo HTLV-1 and HTLV-2 infection in T-lymphocytes. (A): Jurkat or PBLs were transfected either with 2.5 μg of an ADAR1 or of a backbone vector, together with 2.5 μg of a plasmid encoding the luciferase gene under the control of the HTLV-1-LTR or HTLV-2-LTR. Twenty-four hours later, Jurkat (B, C) or PBLs (D, E) were co-cultured with (B, D) irradiated C91-PL (HTLV-1), (C, E) C19 (HTLV-2) or (B, C, D, E) Jurkat (non-infected) cells for 24 hours. Luciferase activity was measured, and results normalized by protein concentration as determined by the Bradford method and calculated as fold change compared to infected cells transfected with the control plasmid arbitrarily set to 100%. (D, E): PBLs were treated with AZT (50 μM) before the co-culture with C91-PL or C19 irradiated cells. (B, C): Data are the mean ± standard deviation (SD) from 3 independent experiments. Asterisks indicate statistically significant differences between treated and untreated cells (paired Student t test, *p < 0.05; **p < 0.01). (D, E): Data are representative of two different experiments obtained with two different blood donors.