Figure 2

HIV-1 Vif destroys A3G cytidine deaminase activity in both cells and virions. 293T cells were cotransfected with HAA3G22K or A3GD128K in combination with HXB2N, HXB2NΔVif or HXB2B3. At 48 h post-transfection, the cells were collected, and virions in culture supernatants were precipitated by ultracentrifugation for Western blot analysis (A). HAA3G22K was cotransfected into 293T cells with HXB2N, HXB2NΔVif or HXB2B3. At 48 h post-transfection, cells and culture supernatants were harvested to measure A3G cytidine deaminase activity (B). A3GD128K was cotransfected into 293T cells with HXB2N, HXB2NΔVif or HXB2B3. At 48 h post-transfection, cells and culture supernatants were harvested to measure A3G cytidine deaminase activity (C).