Figure 4

Triptolide inhibits Tat-mediated LTR activation. (A) TZM-bl cells were co-transfected with pEF1-Tat and pRL-TK, and treated with the test compounds. Luciferase activity was measured at 48 h post-transfection. (B)TZM-bl cells were treated with TNF-α (10 μng/mL) in the presence of the test compounds, and luciferase activity was measured at 24 h post-transfection. (C) Jurkat cells were co-transfected with pLTR-Luc or pLTRΔκB-Luc, pEF1-Tat and pRL-TK in the presence of the test compounds. Luciferase activity was measured 48 h post-transfection. (D) Jurkat cells were co-transfected with pGL3-LTR and pRL-TK in the presence of the test compounds. TNF-α (10 μng/mL) was added 36 h post-transfection. Luciferase activity was measured 48 h post-transfection. Pyrrolidine dithiocarbamate (PDTC) at 100 μM and flavopiridol at 20 nM were used as positive controls. Results were presented as the mean plus the standard deviations (n = 3).