Aggregate complexes of HIV-1 induced by multimeric antibodies

Retrovirology

Table 2 Env-specific antibodies of the IgA class were incubated purified HIV-1 and particle size determined by dynamic light scattering

Assessment of aggregation of HIV-1_BaLby IgA-class mAbs specific for Env
Antibody	Isotype	Virus Aggregation¹	Δ Viral Size (nm)	PDI
2F5	IgG	-	14.6	0.19
	dIgA	++	75	0.63
	pIgM	+	32.1	0.41
b12	IgG	-	12.7	0.27
b12	dIgA	++	63	0.67
ACL4	mIgA	+	26.9	0.29
E10	mIgA	-	17.3	0.36
XA1	mIgA	-	-4.1	0.21

The change in the size of viral particles from virus alone (150.2 nm), and the polydispersity index (PDI) of virus-Ab preparations assesses the presence of aggregates.
¹Aggregate formation was scored by the maximum size measured: ++ for Δ > 50 nm; + for 25 ≤ 50 nm; - for ≤ 25 nm.

ISSN: 1742-4690