Sizing measurements of pre-immunization compared to post-immunization samples for RV144 serum IgA. (A) Scatter plots and (B) paired size measurements for pre and post immunization IgA samples incubated with HIV-1A244. Purified virions and IgA samples were incubated for 1 hour and measured using dynamic light scattering. Each circle shown represents the average of 3 measurements, with experiments performed three times for each sample. Horizontal dashed line indicates the size of untreated viral preparations. Error bars indicate the standard deviation within the measurements for each patient at each visit.