MxB variant | Restriction of HIV-1a | Binding to HIV-1 | Oligomerizationc |
|---|
CA-NC Complexesb |
|---|
WT | + | +++ | + |
Δ (1–20) | - | - | + |
Δ (1–43) | - | - | ND |
Δ (1–60) | - | - | + |
Δ (1–84) | - | - | ND |
Δ (1–102) | - | ND | ND |
Δ (1–387) | ND | - | ND |
Δ (572–715) | - | + | - |
Δ (623–715) | - | + | - |
413-679 | ND | - | ND |
MxB (1–90)-MxA (43–662) | + | +++ | ND |
L661k | - | + | - |
- WT, Wild type.
- ND, Not determined.
- aRestriction was measured by infecting cells expressing the indicated MxB variant with HIV-1-GFP. After 48 hours, the percentage of GFP-positive cells (infected cells) was determined by flow cytometry. “+”: indicates restriction, “-”: indicates absence of restriction. ND: Not Determined.
- bBinding to the HIV-1 capsid complexes was determined for each MxB variant as described in Methods. “+++”: indicates 100% binding which corresponds to the amount of wild type MxB bound to HIV-1 CA-NC complexes, “+”: indicates ~10% binding compared to wild type MxB when expressed at similar levels, “-”: indicates no binding. Experiments were repeated at least three times, and standard deviations are shown in Figure legends.
- cWild type and mutant MxB-FLAG proteins were assayed for association with wild-type MxB-HA as described in Methods. “+”: oligomerization observed in wild-type MxB when expressed at similar levels. ND: Not Determined.
