Figure 4

Variation in amino acid genetic diversity across HIV-1 env . Box plots displaying Mean Average Pairwise Distances (APD) between the (A) paired CSF (red) and plasma (black) variants of subjects with statistical evidence of CSF viral compartmentalization (Compart.) across all regions of HIV-1 env (n = 8) and (B) paired CSF (red) and plasma (black) of variants of subjects without statistical evidence of CSF viral compartmentalization (n = 7). In panel (C) the Mean Between Group (CSF vs. Plasma) APD is shown for subjects with statistical evidence of CSF viral compartmentalization across all regions of HIV-1 env (n = 8) and in (D) the Mean Between Group (CSF vs. Plasma) APD is shown for subjects without statistical evidence of CSF viral compartmentalization across all regions of HIV-1 env (n = 7). All calculations were performed after the exclusion of sequences with statistical evidence of hypermutation. The Wilcoxon matched-pairs signed rank test was used to determine p-values for paired observations. APD = Average Pairwise Distance. For all comparisons, p-values <0.05 are considered significant. * = Statistically significant.