Volume 9 Supplement 1

Abstracts from the 17th International Symposium on HIV and Emerging Infectious Diseases (ISHEID)

Open Access

A new unique recombinant HIV-1 revealed in Belarus

Retrovirology20129(Suppl 1):P32

https://doi.org/10.1186/1742-4690-9-S1-P32

Published: 25 May 2012

Material and methods

Blood plasma, EIA, western blot, RT-PCR, sequencing, SeqScape, BioEdit, Mega4.1, statistica 6.0, software.

Results

In April 2010 we have performed resistance tests of plasma sample obtained from patient Mos, 6 years old girl, born from HIV-infected mother. The phylogenetic analysis of the DNA fragment of patient Mos had shown that sample has been clustered with HIV-1 subtype A on gene pol, but was different from other analyzed samples, subtype A consensus IDU-A and reference sequences (AF004885). The Mos isolate is the most similar to AF413987 from Ukraine (subtype A) the p-distance was 0.066. The comparison of sequences from gag gene p17/p24 region of Mos isolate with reference sequences HIV-1 of subtype A demonstrates that average p-distance was 0.129, and with reference sequences of subtype B was 0.075. Average p-distance on gag gene (the Mos isolate) with CRF03_AB (AF414006.1, Belarus and AF193276.1 CRF03_AB KAL153) was 0.121 if compared with 0.013 p-distance between reference sequences. The analysis of Mos isolate sequences on V3 loop gp120 gene env region HIV-1 has shown that average p-distance with reference isolates subtype B was 0.323, and with A subtype was 0.155. Average p-distance sequence of Mos isolate with reference isolates AF414006.1 and AF193276.1 (CRF-03_AB) was 0.308.

Conclusion

Thus, it has been shown that Mos isolate is a unique recombinant form, but differs in genome structure from the one described earlier CRF03_ AB (AgagBpolBenv). The new recombinant HIV-1 has the following structure: BgagApolAenv. Sequences of new HIV-1 unique recombinant in gag, pol and env genes were submitted to EMBL/Genbank/DDBJ under accession numbers: FR775442.1, FN995656.1, FR775443.1.

Authors’ Affiliations

(1)
Briem

Copyright

© Eremin et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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