Figure 3
From: Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway
APH-2ΔbZIP does not interact with c-Jun and JunB and fails to stimulate their transcriptional activity. (A and B) APH-2ΔbZIP does not bind to c-Jun or JunB, respectively. Nuclear extracts from 293T cells transfected with the indicated vectors were prepared 48 hours post-transfection. Co-immunoprecipitations were then performed with Myc or His antibodies as indicated, followed by Western blotting with the indicated antibodies (WB). (C and D) APH-2ΔbZIP does not stimulate the transcriptional activity of c-Jun and JunB. The pAP-1 luciferase reporter construct was transiently co-transfected with the indicated expression vectors. Cells were lysed 48 hours post-transfection and processed for luciferase assays and Western blot analysis. The luciferase values represent an average of at least three independent experiments.