Figure 1
From: Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway
APH-2 stimulates basal AP-1 transcription through c-Jun, JunB and JunD. 293T cells were transfected with the pAP-1 luciferase construct and the indicated expression vectors. Luciferase and Renilla activities were measured 48 hours after transfection. The average of at least three different experiments is shown. The expression levels of the transfected proteins were analysed by Western blot using the indicated antibodies. (A) HBZ inhibits AP-1 transcription. (B) APH-2 activates AP-1-mediated transcription. (C, D and E) APH-2 enhances the stimulatory effect of c-Jun, JunB and JunD, respectively, on an AP-1 responsive promoter.