Figure 7
Effect of WDO-217 on the interaction of NCp7(11-55) with SL3 or PBS. (A) Effect of WDO-217 on the emission spectra of 3HC-NC(11-55) complexed with ΔP(-)PBS. Emission spectra of 3HC-NC (0.2 μM) in the absence (square) and in the presence of ΔP(-)PBS (0.2 μM)(disk). For monitoring the effect of 10 μM WDO-217, the spectra were recorded immediately after its addition to the complex (triangle) and after 30 minutes (star). (B) Effect of WDO-217 on the emission spectra of 3HC-NCp7(11-55) complexed with SL3 RNA. Conditions and symbols are as in A. (C) Importance of the order of addition of the reactants on the emission spectra of 3HC-NCp7(11-55) complexed with SL3. The emission spectra of 3HC-NC(11-55) (0.2 μM) in the absence (square) and in the presence of WDO-217 (10 μM) (circle). SL3 (0.2 μM) was added to 3HC-NCp7(11-55) (0.2 μM) pre-incubated with WDO-217 (10 μM) for 30 minutes (triangle). Spectra were recorded in 10 mM phosphate buffer, 100 mM NaCl, pH 7.0. Excitation wavelength was 340 nm.